Genetik hastalardan nasıl kurtuluruz?
How is gene deletion done? Gene deletion is the process of removing a specific gene or a portion of a gene from an organism’s DNA. There are several methods for gene deletion, including;
CRISPR/Cas9: This is a popular gene editing tool that uses a specific RNA molecule and an enzyme called Cas9 to cut DNA at a specific location. By designing a guide RNA that targets the specific gene to be deleted, the Cas9 enzyme can be used to cut the DNA and create a double-stranded break, which can then be repaired by the cell’s DNA repair machinery resulting in the deletion of the targeted gene.
Homologous recombination: This method involves using a specially engineered DNA construct that contains a selectable marker and a portion of DNA that is homologous to the target gene. The construct is introduced into cells, and the cells are then subjected to a selection process that favors cells that have incorporated the construct via recombination into their genome, effectively replacing the target gene with the selectable marker.
RNA interference (RNAi): This method involves introducing a small interfering RNA (siRNA) molecule that is complementary to the target gene’s mRNA, which can bind to and degrade the mRNA, resulting in a reduced level of protein expression. With sustained RNAi treatment, the gene expression can eventually be depleted.
The specific method chosen for gene deletion will depend on the experimental design, the organism being studied, and the desired outcome. Additionally, it’s important to note that gene deletion in humans and other complex organisms is still a developing technology, and more research is needed to fully understand the potential risks and benefits of this type of gene editing.
Verification of the edit: Finally, the scientist verifies that the desired gene editing has taken place. This can be done using various techniques, such as PCR or DNA sequencing.
Overall, gene editing is a powerful tool that has the potential to treat genetic diseases and improve agriculture and biotechnology. However, it is important to use it responsibly and with consideration for the ethical implications.
